ABSTRACT
Objective To explore the effects of silencing Livin gene by RNA interference mediated by lentiviral vector on colorectal cancer HT-29 cell xenograft growth and sensitivity to radiotherapy in nude mice.Methods BALB/c nude mice models were established by subcutaneously inoculating differently treated HT-29 cells into nude mice and the tumor growth situation of tumors was observed by measuring the volume of tumors and the weight of the nude mice at different time points after cell seeding.Livin expression was detected by RT-PCR and immunohistochemistry,respetively.Apoptosis rate was detected by TUNEL.Normal saline,lentivirus carring unrelated sequences,lentivirus caning Livin shRNA were injected intratumorally.All the nude mice were given 10 Gy of 6 MV X-ray irradiation.The changes of mice weight and the tumor volume were measured at different time points and the weight and tumor growth curves were drawn.Results The inhibition rate of tumor volume was(50.04 ± 0.07)％ and the tumor weight of the RNA interfering group was significantly less than that in experimental group compared to the blank and negative groups(F=4.85,P＜0.05),and the inhibition rate of tumor weight was(50.27 ±0.17)％.Relative Livin mRNA expression level in the RNA interfering experimental group was(17.75 ±0.08)％,and was significantly lower than that of the blank group(67.60 ± 0.05)％ and the negative group(68.54 ± 0.03)％(F=89.97,P＜0.01).Livin protein expression level in the RNA inferring group was also significantly lower[(36.00 ± 3.40)％ versus(85.00 ± 3.15)％,(80.33 ± 3.08)％,F=107.32,P＜0.01].The apoptosis rate in the RNA interfering experimental group was significantly higher than that in the blank and the negative groups[(23.67 ± 2.25)％ versus(5.00 ± 1.50)％,(8.33 ± 1.82)％,F=56.94,P＜0.01].Combined with radiotherapy,the tumor volume at different groups had significant difference(F=10.70,P＜0.01),and RNA interfering group was significantly less than negative group and blank group(F=7.01-9.32,P＜0.01).Conclusions Silencing of Livin gene expression by lentiviral vector-mediated RNA interference could inhibit the growth of colorectal HT-29 cell xenograft and increase the sensitivity of the transplanted tumors to radiotherapy.
ABSTRACT
Objective To further know the Reiter′s syndrome of venerel type.Methods Two patients with Reiter′s syndrome of venereal type were reported and the related literature was reviewed.Results Features of the disease were described in detail,including its etiology,pathogenesis,clinical feature,diagnosis and differential diagnosis.Conclusion Reiter′s syndrome of venereal type is caused by C.trachmatis and U.urealyticum ,etc,infection.It is featured with urethritis,arthritis and conjunctivitis.In addition,the other related clinical features include mucocutaneous,genital system,cardiovascular system and nervous system damages.Its pathogenesis is associated with hereditary susceptibility and HLA B27 antigen.Therefore,the Reiter′s syndrome of venereal type should be considered when urethra,joint,conjunctiva and mucocutaneous damages were expressed after sexually transmitted disease.
ABSTRACT
Objective To evaluate the clinical and X-ray features of bone lesion in early congenital syphilis. Methods Between 1997 and 2005, 48 cases of early congenital syphilis were diagnosed. Among those 48 cases, there were 16 cases with bone lesions detected. The clinical and radiographic features of these 16 cases of bone lesion in early congenital syphilis were retrospectively analyzed. There were 10 males and 6 females with an age between 25-57 d. All patients were confirmed with serology and radiographs of limbs. They were given Penicilin G of 15-20 WU?kg-1?d-1, 3 times a day in a total of 14 days for one course of treatment. Results Rapid plasma reagent test(RPR) of all the cases was positive and they were 3 cases with 1∶128, 2 cases with 1∶64, 6 cases with 1∶32, 3 cases with 1∶16 and 2 cases with 1∶8. All the cases were positive for TPPA. 30 parents of 16 cases were tested for RPR and TPPA. The results showed that 16 mothers were positive for RPR, 13 fathers were positive for RPR. All the parents were positive for TPPA, and negative for HIV test. The main clinical features included active disorder, swelling of involved limbs, malnutrition, light body weight and multi-organ functional lesion. The main X-ray features were metaphysitis, periostitis and osteomyelitis involving all of the long bones of the extremities. Widen of the junction between epiphysis and diaphysis was the characteristic features of early congenital syphilis. After treatment, RPR became negative and sclerotic lesion was recovered and only remain periosteum proliferation. Hutchinson teeth were seen in some patients. Conclusion Bone lesions in early congenital syphilis has its characteristic clinical and radiographic features. The bone lesion could be cured after effective treatment.